OLA1 Knockout cell line (L-02)

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OLA1 Knockout cell line (L-02) (Hela-contaminated cells, not for sales) Order

Catalog#

YKO-H869

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

OLA1 Knockout cell line (L-02)

Cell Morphology

Epithelial-like, adherent

Passage ratio

1:2~1:4

Species

Human

Gene Name

OLA1

Gene ID

29789

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Culture method

80% RPMI-1640+20% FBS

Accession

CVCL_E0X6(Cellosaurus)

Target region

Knockout region: Exon 5 (based on transcript OLA1-201)
Validation: PCR+Sanger Sequencing

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:L-02			STR Info (Cell bank) Cell Line:L-02
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	11	12		11	12
D13S317	13.3	13.3		13.3	13.3
D7S820	12	12		12	12
D16S539	9	10		9	10
VWA	16	16		16	18
TH01	7	7		7	7
AMEL	X	X		X	X
TPOX	12	12		12	12
CSF1PO	10	10		10	10
D12S391	20	20
FGA	18	21
D2S1338	17	17
D21S11	27	28
D18S51	16	16
D8S1179	12	12
D3S1358	15	18
D6S1043	18	18
PENTAE	7	17
D19S433	13	13
PENTAD	8	15
D1S1656	12	15

* Hela-contaminated cells, not for sales

Reviewed by ICLAC (ref: 190616). L-02 was reported to be established from normal liver cells but its STR profile corresponds to HeLa. Samples tested in this study were directly deposited in the cell line repository that performed the investigation. L-02 has a derivative: HL-7702BaPT. No STR profiling information was available for the derivative at the time of review.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the OLA1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the L-02 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, OLA1 gene knockout OLA1 Knockout cell line (L-02) will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

OLA1 Knockout cell line (L-02)

Catalog#

YKO-H869

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

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OLA1 Knockout cell line (L-02)

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